Gu Zhujun was born in Haimen, Jiangsu Province, in 1933. In 1954, he graduated from the Shanghai Branch of the Central Art Academy and in 1963, he graduated from the Oil Painting research class of the same Academy. He headed the Design Office of the Tianjin People's Fine Arts Publishing House and later became editorial advisor of the journal Chinese Oil Painting [中国油画].

Gu excels in oil painting. He collaborated with Wang Yongyang.